MITORIBOSOMES FROM CANDIDA UTILIS
نویسندگان
چکیده
منابع مشابه
Mitoribosomes from Candida Utilis
Highly purified mitochondrial ribosomes (mitoribosomes) have been obtained from the yeast Candida utilis. Sedimentation analysis in sucrose gradients made in 5 mM MgCl(2), 1 mM Tris, pH 7.4 and 50 mM KCl clearly distinguishes mitoribosomes (72S) from cytoplasmic ribosomes (cytoribosomes) (78S). Mitoribosomes are completely dissociated into 50S and 36S subunits at 10(-4)M MgCl(2) whereas complet...
متن کاملThe preparation of crystalline transaldolase from Candida utilis.
* Aided by grants from the National Institute of Arthritis and Metabolic Diseases (Grant A-1845) of the U.S. Public Health Service, and the Rockefeller Foundation. 1 Abbreviations: RNA, ribonucleic acid; the capital letters A, U, and C are used for the nucleotides adenylic, uridylic, and cytidylic acid, respectively, or their corresponding residues in polynucleotide chains; ADP, UDP, and CDP, t...
متن کاملUrea amidolyase. I. Properties of the enzyme from Candida utilis.
Candida utilis contains an inducible enzyme, urea amidolyase, which catalyzes the decomposition of urea with formation of carbon dioxide and ammonia. This reaction is accompanied by the cleavage of ATP with liberation of equimolar amounts of ADP and inorganic phosphate, is strictly dependent for activity on divalent (Mg++ or Mn++) and monovalent (K+, Rb+, Csf, or NH4+) cations, and requires cat...
متن کاملSCP production from Candida utilis isolated from Vellar estuary
In the present study a total of 65 morphologically distinct yeast strains isolated from water (30) and sediment (35) samples collected from Vellar estuary (Lat 1129’24”N and Long. 7945’ 36” E). From water 30yeast strains and from sediment 35 strains were obtained. The most potent isolate VEW12 was selected for further study and was identified as C. utilis. Optimization of growth revealed that p...
متن کاملPurification and characterization of phospholipase B from Candida utilis.
Phospholipase B (PLB) from the asporogenous yeast Candida utilis was purified to homogeneity from a culture broth. The apparent molecular mass was 90-110 kDa by SDS-PAGE. The enzyme had two pH optima, one acidic (pH 3.0) and the other alkaline (pH 7.5). At acidic pH the enzyme hydrolyzed all phospholipids tested without metal ions. On the other hand, the PLB showed substrate specificity and req...
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ژورنال
عنوان ژورنال: Journal of Cell Biology
سال: 1972
ISSN: 1540-8140,0021-9525
DOI: 10.1083/jcb.54.3.468